NK-Cell Serum-free Media

Our specialized cell culture media support the growth, expansion, and functional maintenance of NK cell for research, cell therapy development, and bioprocessing applications. Serum-free formulations help improve consistency, reduce variability, and streamline downstream workflows.

How to Choose an NK-Cell Serum-Free Medium

Choose the medium according to your culture and activation strategy:

  • P01 series: Select the P01 medium for standard NK-cell activation and expansion workflows. It supports NK cells derived from PBMCs, cord blood mononuclear cells, iPSCs, and NK-92 cells.
  • NE01 series: Select the phenol-red-free NE01 medium for imaging, color-sensitive assays, or manufacturing processes requiring a phenol-red-free formulation.
  • Complete Kit: Choose a P01 Complete Kit or NE01 Complete Kit when both activation and expansion components are required. Each complete kit includes basal medium, supplement, and Cytokines I, II, and III.
  • Basic Kit: Choose a P01 Basic Kit or NE01 Basic Kit for previously activated NK cells or when using your own activation, feeder-cell, or cytokine system. The basic kit includes basal medium, supplement, and Cytokine III and is primarily intended for the expansion stage.

Both series support NK cells derived from PBMCs, cord blood, iPSCs, and compatible NK cell lines. For a new process, compare suitable configurations using expansion, viability, purity, phenotype, and cytotoxic activity.

Catalog NumberDescriptionDatasheetMSDS
NE000-N021OptiVitro® NK Cell Expansion Serum-free Medium Kit P01DownloadDownload
NE000-N031OptiVitro® NK Cell Expansion Serum-free Basic Kit P01DownloadDownload
NE000-N042OptiVitro® NK Cell Expansion Serum-free Medium Kit NE01 (phenol red-free)DownloadDownload
NE000-N052OptiVitro® NK Cell Expansion Serum-free Basic Kit NE01 (phenol red-free)DownloadDownload